Vitamins are essential to maintenance of healthy homeostasis, to catalyze biochemical reactions and aid in metabolic functions in humans. Deficiency in vitamins or excess can cause health problems such as cardiovascular disease, kidney disease, depression, and anemia. Therefore, there is a great need to monitor their concentration in plasma and other biological fluids as a means of diagnosis of different diseases and evaluation of treatment therapies. The majority of methods used for determination of vitamins in biofluids are chromatographic, such as high-performance liquid chromatography (HPLC). Over the last decade, LC-MS/MS methods have been heavily used for quantification of vitamins in complex matrices (e.g. plasma, serum, urine, breast milk, or amniotic fluid) due to their high sensitivity, selectivity, wide dynamic range of concentrations, and measurement of all forms of vitamins in human biofluids.
There are two groups of vitamins: water-soluble and fat-soluble vitamins. Water-soluble vitamins include ascorbic acid (vitamin C) and B group vitamins: thiamine (B1), riboflavin (B2), niacin (B3), pantothenic acid (B5), pyridoxal (B6), folic acid (B9), and cyanocobalamin (B12) . Fat-soluble vitamins include retinol (vitamin A), tocopherol (vitamin E), radiostol (vitaminD), and vitamin K. The measurement of vitamins in human biological fluids is important for evaluation of vitamin status, disorders of lipid malabsorption, diagnosis of anemia or hypervitaminosis, toxicity assessment and various other purposes. Vitamins are also useful indicators, especially in clinical, nutritional and metabolomics studies.
Biomics group can undertake the analysis of selected vitamins or groups of vitamins in various matrices using validated methods.